The Nun, a spiritual prequel based on a character introduced in The Conjuring 2, was released in 2024. The plot focused on the origins of the demonic nun Valak before coming in contact with the Warrens.

Sister Irene and Lorraine Warren are revealed to be part of the same bloodline, explaining why both characters are targeted by demons like Valak and Annabelle. The characters played by Taissa and Vera Farmiga are also related in the franchise.